![]() Incubation conditions determine which bacteria can grow and thus beĬounted. Scientific notation is routinely used in calculations. Use of extremely small dilutions and extremely large numbers of cells, Since the enumeration of microorganisms involves the Counting plates should have 30-300Ĭolonies at least. Number multiplied by the dilution factor to find out concentration ofĬells in the original sample. Total number of colonies is counted and this It is assumed that each colony arises fromĪn individual viable cell. Multiplication of aīacterium on solid media results in the formation of a macroscopicĬolony visible to naked eye. Plates are incubated so that colonies are formed. ![]() The suspension is spread over the surface of growth medium. Dilution procedure influences overall counting (also called a Standard Plate Count) is one of the most common methods,įor enumeration of bacteria. Direct counts of bacteria requireĪ dye to be introduced to the populations of bacteria to allow This enables an estimate to be establishedįor quantifying the bacteria. To a point at which the number of bacteria has been reduced to Some of the methods used involve diluting the sample So small and numerous, counting them directly can be veryĭifficult. ![]() Useful in many aspects of industrial microbiology. Important in dairy microbiology, food microbiology, and waterĬount in drinking water, fresh milk, buttermilk, yogurt, can be Enumeration of microorganisms is especially Having to compare the amount of bacterial growth under variousĬonditions. To determine the number of bacteria in a given sample as well as ![]() Part of daily routine, the laboratory microbiologist often has ![]()
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